Difference between revisions of "2017 Feb Taeyoung Lab note"

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(2017.2.7)
(PCR with LIP short primer)
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1% agar, 100V, 1h, Tm:56,
 
1% agar, 100V, 1h, Tm:56,
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===Primer re-design===
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SLIP-13 and 16 is re-designed
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python ~/py/LIP/6-1.manual_primer_design.py CS-14-contigs.fa.blastn.single_type.blastn.indel_candidates.read_evidence ../../CS-14/qvalue/CS-14-contigs.fa > CS-14.short.primer.fa.2candidates

Revision as of 06:39, 7 February 2017

Contents

2017.2.2

PCR with LIP short primer

Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order

LIP01-24

Last 4 bands are Tubulin control in CS-12,CS-14,KS-04,W82

1% agar, 100V, 1h, Tm:56,

2017.2.3

PCR with LIP short primer

Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order

SLIP01,02,03,06,07,09,13,14,15,16,20,24

Last 3 bands are Tubulin control in CS-12,CS-14,W82

1% agar, 100V, 1h, Tm:56,

2017.2.6

PCR with LIP short primer

Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order

SLIP01,02,03,06,07,13,14,15,16,20,24

Last 3 bands are Tubulin control in CS-12,CS-14,W82

1% agar, 100V, 1h, Tm:56,

2017.2.7

PCR with LIP short primer

Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order

2017.02.07 SLIP-08,12,13,20 with Wild and W82

Last 3 bands are Tubulin control in CS-12,CS-14,W82

1% agar, 100V, 1h, Tm:56,

Primer re-design

SLIP-13 and 16 is re-designed

python ~/py/LIP/6-1.manual_primer_design.py CS-14-contigs.fa.blastn.single_type.blastn.indel_candidates.read_evidence ../../CS-14/qvalue/CS-14-contigs.fa > CS-14.short.primer.fa.2candidates