Difference between revisions of "2017 Jayern Lab note"
From Crop Genomics Lab.
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tophat2 -p 1 -G Vradiata.gff Vradiata SunhwaN_root_1.fastq.gz SunhwaN_root_2.fastq.gz | tophat2 -p 1 -G Vradiata.gff Vradiata SunhwaN_root_1.fastq.gz SunhwaN_root_2.fastq.gz | ||
+ | (Total 34 RNA paired end seq) |
Revision as of 05:47, 17 March 2017
Contents |
03/02
perl deconseq.pl -f 11_180bp_1.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f 11_180bp_2.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f 4_180bp_1.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f 4_180bp_2.fastq -dbs chloro -i 90 -c 90
03/03
perl deconseq.pl -f V_nakashimae_2_1.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f V_nakashimae_2_2.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f V_nepal_1_1.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f V_nepal_1_2.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f V_nipp_1_1.fastq -dbs chloro -i 90 -c 90 perl deconseq.pl -f V_nipp_1_2.fastq -dbs chloro -i 90 -c 90
03/06
python PE-pairing.py filtered.V_nakashimae_2_1.fq filtered.V_nakashimae_2_2.fq python PE-pairing.py filtered.V_nepal_1_1.fq filtered.V_nepal_1_2.fq python PE-pairing.py filtered.V_nipp_1_1.fq filtered.V_nipp_1_2.fq
bwa index Vreflexo.fasta
03/07
<< SSR marker design >> perl gmat.pl -r 5 -m 2 -x 10 -s 0 -i Vradiata.fa python 01.ssr.get_seq.py Vradiata.fa.ssr python 02.p3in.split_nParts.py Vradiata.fa.ssr.p3in
03/08
primer3_core < Vradiata.fa.ssr.p3in > Vradiata.fa.ssr.p3out
03/10
python parsing_primer3_output.py Vradiata.fa.ssr.p3out > Vradiata.fa.ssr.p3out.post pyton post.parse_into_gff.py Vradiata.fa.ssr.p3out.post > Vradiata.fa.ssr.p3out.post.gff
03/15
bowtie-build Vradiata.fa Vradiata bowtie-build Vreflexo.fa Vreflexo bowtie-build Vangularis.fa Vangularis
03/16
tophat2 -p 1 -G Vradiata.gff Vradiata 1_1.fastq.gz 1_2.fastq.gz tophat2 -p 1 -G Vradiata.gff Vradiata 2_1.fastq.gz 2_2.fastq.gz tophat2 -p 1 -G Vradiata.gff Vradiata 4_1.fastq.gz 4_2.fastq.gz . . . tophat2 -p 1 -G Vradiata.gff Vradiata SunhwaN_root_1.fastq.gz SunhwaN_root_2.fastq.gz (Total 34 RNA paired end seq)