Difference between revisions of "2018 June Taeyoung Lab note"

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Line 2: Line 2:
 
  library(UpSetR)
 
  library(UpSetR)
 
  upset(Data,sets = c("Lus","Ptr","Rco","Jcu","Mes","Hbr"),order.by="freq",empty.intersections="on")
 
  upset(Data,sets = c("Lus","Ptr","Rco","Jcu","Mes","Hbr"),order.by="freq",empty.intersections="on")
 +
 +
==pheatmap==
 +
library(pheatmap)
 +
Data<-read.table("PE_gene_list.txt.RPKM",header=T)
 +
head(Data)
 +
 +
            Gene        B        Y        G        I
 +
1 Jatcu.01g001630  0.777053  0.900879  1.93941  4.831030
 +
2 Jatcu.02g000639  3.246920  3.148340 23.04090 35.914500
 +
3 Jatcu.02g000941 12.631700 13.201900 23.73470 14.621300
 +
4 Jatcu.02g000968  7.888650  3.338680  4.60163 10.876700
 +
5 Jatcu.02g001631  5.646930  7.952740  4.64940  5.600850
 +
6 Jatcu.03g000784  3.271440  3.703690  1.34210  0.956547
 +
 +
rownames(Data)=Data$Gene
 +
pheatmap(log(Data[2:5]+1),color = colorRampPalette(c("white","firebrick3"))(50),cluster_cols = FALSE, cluster_rows = FALSE)

Revision as of 07:32, 14 June 2018

UpSetR plot

library(UpSetR)
upset(Data,sets = c("Lus","Ptr","Rco","Jcu","Mes","Hbr"),order.by="freq",empty.intersections="on")

pheatmap

library(pheatmap)
Data<-read.table("PE_gene_list.txt.RPKM",header=T)
head(Data)
            Gene         B         Y        G         I

1 Jatcu.01g001630 0.777053 0.900879 1.93941 4.831030 2 Jatcu.02g000639 3.246920 3.148340 23.04090 35.914500 3 Jatcu.02g000941 12.631700 13.201900 23.73470 14.621300 4 Jatcu.02g000968 7.888650 3.338680 4.60163 10.876700 5 Jatcu.02g001631 5.646930 7.952740 4.64940 5.600850 6 Jatcu.03g000784 3.271440 3.703690 1.34210 0.956547

rownames(Data)=Data$Gene
pheatmap(log(Data[2:5]+1),color = colorRampPalette(c("white","firebrick3"))(50),cluster_cols = FALSE, cluster_rows = FALSE)