Antibiotics

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Working Concentration

Antibiotics	Stringent plasmids	Relaxed Plasmids
Ampicillin	20 ug/ml	50 ug/ml
Carbenicillin	20 ug/ml	60 ug/ml
Chloramphenicol	25 ug/ml	170 ug/ml
Kanamycin	10 ug/ml	50 ug/ml
Streptomycin	10 ug/ml	50 ug/ml
Tetracycline	10 ug/ml	50 ug/ml

Reference

Molecular cloning volume 3 third edition, Sambrook and Russel, Cold Spring Harbor Laboratory Press,2001

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