BZIP study

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Contents

Identification

Pfam-based approach

HMM domain clans

Regarded as the main domain for bZIP transcription factor. Clearly distinguishes basic region and leucine zipper.

Used for further analysis.

Conserved basic region, weaker leucine zipper.

  • bZIP_Maf (PF03131)

No distinct basic region. No clear leucine zipper interface.

Downloading and generating HMM consensus sequence file

  1. Download Stockholm alignment file from Pfam bZIP_1 > Alignment > Download options > Seed

Seed: the curated alignment from which the HMM for the family is built

  1. Use 'hmmbuild' command from HMMER software to convert Stockholm alignment into a profile HMM
hmmbuild [options] hmmfile alignfile

hmmbuild reads a multiple sequence alignment file alignfile , builds a new profile HMM, and saves the HMM in hmmfile.