Heat shock

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Protocol for Transformation

Reaction Protocol

  1. Prepare competent cell(100ul aliquot) + 10ul Vector with insert gene
  2. Mixture tube(110ul) in ice 20 min for ligation
  3. Heat shock at 42℃ 1 min 30 sec
  4. Quenching in ice shortly
  5. add SOC broth media 600ul
  6. Incubation at 37℃
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