Primer3

SEQUENCE_ID=

SEQUENCE_TEMPLATE=

SEQUENCE_TARGET=4084,9

PRIMER_TASK=pick_pcr_primers

PRIMER_PICK_LEFT_PRIMER=1

PRIMER_PICK_INTERNAL_OLIGO=1

PRIMER_PICK_RIGHT_PRIMER=1

PRIMER_OPT_SIZE=20

PRIMER_MIN_SIZE=18

PRIMER_MAX_SIZE=22

PRIMER_MAX_NS_ACCEPTED=1

PRIMER_PRODUCT_SIZE_RANGE=509-1009

P3_FILE_FLAG=1

SEQUENCE_INTERNAL_EXCLUDED_REGION=

PRIMER_EXPLAIN_FLAG=1

=