Difference between revisions of "Sugar analysis"

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(Created page with "== '''<Sugar analysis protocol> ''' == 1. Grind seeds to a fine powder using a coffee bean grinder, then pass through a 100-㎛ standard testing sieve. 2. Weigh a 1g sample a...")
 
 
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== '''<Sugar analysis protocol>
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== '''<Sugar analysis protocol>''' ==
''' ==
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1. Grind seeds to a fine powder using a coffee bean grinder, then pass through a 100-㎛ standard testing sieve.
 
1. Grind seeds to a fine powder using a coffee bean grinder, then pass through a 100-㎛ standard testing sieve.
  

Latest revision as of 04:05, 15 March 2018

<Sugar analysis protocol>

1. Grind seeds to a fine powder using a coffee bean grinder, then pass through a 100-㎛ standard testing sieve.

2. Weigh a 1g sample and dissolve the sample in 10mL of 80% ethanol.

3. Vortexing

4. Sonicate sample 1 hour (SPC, B1)

5. Vortexing

6. Shake the sample horizontally for overnight

7. Vortexing

8. Wait a minute for separating supernatant and debris

9. Transfer the 4mL the supernatant to a fresh tube.

10. Filter out the supernatant through a 0.2-㎛ filter.