Difference between revisions of "Revio seq"
From Crop Genomics Lab.
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minimap2 로 mapping Gmax ver2 fa indexing 한 후 | minimap2 로 mapping Gmax ver2 fa indexing 한 후 | ||
| − | minimap2 -x map-pb -t 10 -a /data/ref/Gmax/assembly/Gmax_275_v2.0.fa SS2-2.hifi_reads.fastq | samtools sort -o SS2-2_hifi_re.bam --write-index | + | minimap2 -x map-pb -t 10 -a /data/ref/Gmax/assembly/Gmax_275_v2.0.fa SS2-2.hifi_reads.fastq | samtools sort -o SS2-2_hifi_re.bam --write-index |
-a 옵션 넣어줘야 sam/bam | -a 옵션 넣어줘야 sam/bam | ||
| + | |||
| + | samtools index SS2-2_hifi_re.bam | ||
Revision as of 07:15, 23 May 2024
bioconda 깔았음
barsh 에 export 추가했음, path 설정안돼있으면 source ~/.barsh
minimap2 로 mapping Gmax ver2 fa indexing 한 후
minimap2 -x map-pb -t 10 -a /data/ref/Gmax/assembly/Gmax_275_v2.0.fa SS2-2.hifi_reads.fastq | samtools sort -o SS2-2_hifi_re.bam --write-index
-a 옵션 넣어줘야 sam/bam
samtools index SS2-2_hifi_re.bam
