Difference between revisions of "2017 Feb Taeyoung Lab note"
From Crop Genomics Lab.
(→2017.2.7) |
(→PCR with LIP short primer) |
||
Line 52: | Line 52: | ||
1% agar, 100V, 1h, Tm:56, | 1% agar, 100V, 1h, Tm:56, | ||
+ | |||
+ | ===Primer re-design=== | ||
+ | SLIP-13 and 16 is re-designed | ||
+ | |||
+ | python ~/py/LIP/6-1.manual_primer_design.py CS-14-contigs.fa.blastn.single_type.blastn.indel_candidates.read_evidence ../../CS-14/qvalue/CS-14-contigs.fa > CS-14.short.primer.fa.2candidates |
Revision as of 06:39, 7 February 2017
Contents |
2017.2.2
PCR with LIP short primer
Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order
LIP01-24
Last 4 bands are Tubulin control in CS-12,CS-14,KS-04,W82
1% agar, 100V, 1h, Tm:56,
2017.2.3
PCR with LIP short primer
Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order
SLIP01,02,03,06,07,09,13,14,15,16,20,24
Last 3 bands are Tubulin control in CS-12,CS-14,W82
1% agar, 100V, 1h, Tm:56,
2017.2.6
PCR with LIP short primer
Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order
SLIP01,02,03,06,07,13,14,15,16,20,24
Last 3 bands are Tubulin control in CS-12,CS-14,W82
1% agar, 100V, 1h, Tm:56,
2017.2.7
PCR with LIP short primer
Ladder, 1-wild,1-cultivated,2-wild,2-cultivated order
2017.02.07 SLIP-08,12,13,20 with Wild and W82
Last 3 bands are Tubulin control in CS-12,CS-14,W82
1% agar, 100V, 1h, Tm:56,
Primer re-design
SLIP-13 and 16 is re-designed
python ~/py/LIP/6-1.manual_primer_design.py CS-14-contigs.fa.blastn.single_type.blastn.indel_candidates.read_evidence ../../CS-14/qvalue/CS-14-contigs.fa > CS-14.short.primer.fa.2candidates