Difference between revisions of "GBS"
From Crop Genomics Lab.
(Created page with "== Methods == zcat 4_NoIndex_L004_R1_001.fastq.gz | fastx_barcode_splitter.pl --bcfile barcode.1 --suffix ".fq" --prefix ./4_. --bol --exact mv 4_.unmatched.fq Full.fq /data1/...") |
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== Methods == | == Methods == | ||
zcat 4_NoIndex_L004_R1_001.fastq.gz | fastx_barcode_splitter.pl --bcfile barcode.1 --suffix ".fq" --prefix ./4_. --bol --exact | zcat 4_NoIndex_L004_R1_001.fastq.gz | fastx_barcode_splitter.pl --bcfile barcode.1 --suffix ".fq" --prefix ./4_. --bol --exact | ||
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mv 4_.unmatched.fq Full.fq | mv 4_.unmatched.fq Full.fq | ||
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/data1/program/NGSQCToolkit_v2.3.3/Trimming/TrimmingReads.pl -l 4 -i 4_.BA01.fq | /data1/program/NGSQCToolkit_v2.3.3/Trimming/TrimmingReads.pl -l 4 -i 4_.BA01.fq | ||
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+ | == GBS lecture pipeline == | ||
+ | [[File:GBS pre-processing.pptx|thumbnail]] |
Latest revision as of 04:36, 2 July 2018
Methods
zcat 4_NoIndex_L004_R1_001.fastq.gz | fastx_barcode_splitter.pl --bcfile barcode.1 --suffix ".fq" --prefix ./4_. --bol --exact
mv 4_.unmatched.fq Full.fq
/data1/program/NGSQCToolkit_v2.3.3/Trimming/TrimmingReads.pl -l 4 -i 4_.BA01.fq