Difference between revisions of "HISAT2 pipeline"
From Crop Genomics Lab.
(Created page with "==mapping == /data1/program/hisat2/hisat2-2.2.1/hisat2 -q --phred33 -x /data1/ref/Gmax_275_v2.0.fa.hisat --summary-file SS2-2_1_hisat_summary -p 6 -1 {forward_input.fq.gz} -2...") |
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==mapping == | ==mapping == | ||
/data1/program/hisat2/hisat2-2.2.1/hisat2 -q --phred33 -x /data1/ref/Gmax_275_v2.0.fa.hisat --summary-file SS2-2_1_hisat_summary -p 6 -1 {forward_input.fq.gz} -2 {reverse_input.fq.gz} | samtools view -bS -q30 > {output}.bam | /data1/program/hisat2/hisat2-2.2.1/hisat2 -q --phred33 -x /data1/ref/Gmax_275_v2.0.fa.hisat --summary-file SS2-2_1_hisat_summary -p 6 -1 {forward_input.fq.gz} -2 {reverse_input.fq.gz} | samtools view -bS -q30 > {output}.bam | ||
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Revision as of 06:06, 14 September 2020
mapping
/data1/program/hisat2/hisat2-2.2.1/hisat2 -q --phred33 -x /data1/ref/Gmax_275_v2.0.fa.hisat --summary-file SS2-2_1_hisat_summary -p 6 -1 {forward_input.fq.gz} -2 {reverse_input.fq.gz} | samtools view -bS -q30 > {output}.bam
