Difference between revisions of "SK met"
From Crop Genomics Lab.
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# bwa index <ref.fa> | # bwa index <ref.fa> | ||
# cat SK_met.list | parallel --gnu -j 3 "bwa mem Vradi_ver6.fa ./raw_data/{}_1.fq.gz ./raw_data/{}_2.fq.gz | samtools view -bS -q 30 -> {}.bam" (1.5일) | # cat SK_met.list | parallel --gnu -j 3 "bwa mem Vradi_ver6.fa ./raw_data/{}_1.fq.gz ./raw_data/{}_2.fq.gz | samtools view -bS -q 30 -> {}.bam" (1.5일) | ||
− | # samtools sort/index/ bam samtools faidx ref | + | # samtools sort/index/ {bam} samtools faidx ref |
− | # /data/haggui$ /data/haggui/samtools-1.3.1/samtools mpileup -f Vradi_ver6.fa -v -t DP,AD,ADF,ADR,SP,INFO/AD,INFO/ADF,INFO/ADR -u -b bamfile.list| bcftools call -v -m -O v > SK_met_variant.vcf | + | # /data/haggui$ /data/haggui/samtools-1.3.1/samtools mpileup -f Vradi_ver6.fa -v -t DP,AD,ADF,ADR,SP,INFO/AD,INFO/ADF,INFO/ADR -u -b {bamfile.list}| bcftools call -v -m -O v > SK_met_variant.vcf |
− | # samtools depth sorted.bam > .dep | + | # samtools depth {sorted.bam} > {.dep} |
# wc -l *.dep | # wc -l *.dep | ||
− | # cat | + | # cat {sort.bam.dep} |awk '{sum += $3} END { print sum}' |
Revision as of 01:34, 28 January 2019
materials
raw_data : /NGS/NGS/VignaRadiata/DNA/SK/ /mount_test/sdd1/Mungbean_assemble/ver6/SNP/Vradi_ver6.reseqKJ.sorted.bam
SK010 | TN1509D0657--TCCGGAGA-AGGCTATA |
SK068 | TN1509D0710--TAATGCGC-TAAGATTA |
SK186 | TN1510D0932--TCTCGCGC-TCAGAGCC |
SK049 | TN1509D0695--GAATTCGT-ACGTCCTG |
SK094 | TN1509D0736--TCTCGCGC-GTCAGTAC |
SK152 | TN1510D0898--CTGAAGCT-GCCTCTAT |
SK176 | TN1510D0922--TCCGCGAA-GCCTCTAT |
SK156 | TN1510D0902--CTGAAGCT-TAAGATTA |
- 244 > 63 /data/haggui/raw_data/ 에 sample#.fq.gz 로 저장함
ref : 244 /mount_test/sdd1/Mungbean_assemble/ver6/SNP/Vradi_ver6.fa
- 244 > 63 data/haggui/Vradi_ver6.fa
process
- bwa index <ref.fa>
- cat SK_met.list | parallel --gnu -j 3 "bwa mem Vradi_ver6.fa ./raw_data/{}_1.fq.gz ./raw_data/{}_2.fq.gz | samtools view -bS -q 30 -> {}.bam" (1.5일)
- samtools sort/index/ {bam} samtools faidx ref
- /data/haggui$ /data/haggui/samtools-1.3.1/samtools mpileup -f Vradi_ver6.fa -v -t DP,AD,ADF,ADR,SP,INFO/AD,INFO/ADF,INFO/ADR -u -b {bamfile.list}| bcftools call -v -m -O v > SK_met_variant.vcf
- samtools depth {sorted.bam} > {.dep}
- wc -l *.dep
- cat {sort.bam.dep} |awk '{sum += $3} END { print sum}'